Table 1.

Primers used for PCR amplification of feline retrovirus (FFV, FIV and FeLV) genome sequences.

Virus	Genomic Region	Name	Sense	Primer Sequence (5′-3′)	Reference
FFV	LTR	FFV LTR out	1st forward	TGCACAGGAAGCTCCTTTAGGGTA	Designed herein
		789(rev)	1st and 2nd reverse	TCCCCACGTGTAGAGAAACACCACTC	[43]
		788(fow)	2nd forward	TGTACGGGAGCTCTTCTCACAGACTTGGC	[43]
	glycoprotein/	FFV_F1	1st forward	CCTCGGGTCCAAACACAGCC	Designed herein
	polymerase	FFV_R1	1st reverse	CCAAGGTACATCTCCAGG	Designed herein
	(gag/pol)	FUV2610s	2nd forward	AACAGCAACACTCTGATGTTCCCG	[44]
		FUV3107a	2nd reverse	ATATACATCTCCTTCCTGCGTTCC	[44]
	integrase (int)	SIF5N_mod a	1st forward	TACATGGTTATACCCCACKAAGGCTC	[10]
		SIR1NN	1st and 2nd reverse	GTTTTATYTCCYTGTTTTTCCTYTCCA	[10]
		SIP4N	2nd forward	TGCATTCCGATCAAGGATCAGCATT	[10]
	envelope	env-f2	1st forward	GCTACTTCTACTAGAATAATGTTTTGGATA	[45]
		env-r2	1st reverse	AGCCACAGTAGTAATTGCATTGGCCAGGCC	[45]
		env-f3	2nd forward	GCTTTCAAAAATATGGACATTGTTATGTTA	[45]
		env-r3	2nd reverse	GTTTCTCCAAAATCTGCAAGCATATGGATG	[45]
FIV	reverse	RT out F	1st forward	GGAGTAGGAGGAGGAAAAAGAGGAAC	[46]
	transcriptase (RT)	RT out R	1st reverse	GCCCATCCACTTATATGGGGGC	[46]
		RT int F	2nd forward	GGGCCTCAGGTAAAACAGTGGC	[46]
		RT int R	2nd reverse	GTCTTCCGGGGTTTCAAATCCCCAC	[46]
FeLV	envelope	Env Fow (1689 bp)	Forward	TCTATGTTAGGAACCTTAACCGATG	[47]
		Env Rev (1689 bp)	Reverse	CAGAATATCTGTGGTAC AAGCCTTAA	[47]
		Env Fow (437 bp)	Forward sequencing	GCYTGGTGGGTCTTAGGAA	[47]
		Env Rev (437 bp)	Reverse sequencing	AACARAAGTAAAGACTGTTGG	[47]

^a The last four bases were removed from the original primer sequence (TACATGGTTATACCCCACKAAGGCTCCTCC).