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. 2018 Oct 24;8(4):99. doi: 10.3390/bios8040099

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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LOPAC library pilot screens for 2-color SERCA2a in live cells: (A) Histograms show distribution of observed lifetime changes from single screens, each fit to a Gaussian distribution to determine the SD (~8 ps for G/R and 11 ps for O/M). (B) Peak intensity (mV) for false-positive hit flagging. Bottom: lifetime change in a single 1536-well screen for G/R (C) and O/M (D). Known SERCA inhibitor thapsigargin (10 μM) shown by arrow. Reproducible hits (blue) were identified in three separate screens.