Figure 2.

Standardization of the two-tube multiplex qPCR. (a) Serial dilutions of standard plasmids (10⁶–10¹ copies) were used to construct standard curves. The number of copies detected for each standard plasmid dilution was plotted against the average of the cycle threshold. Efficiencies and coefficient of determination R² values (predictability) are shown in Table 2. (b) Correlation of viral copy numbers detected in multiplex vs. single assays. Correlation coefficients (R) are shown in Table 2.