Table 3.
Taxonomy enrichment results. Repeated measures ANOVA with Bonferroni FWER ≤ 0.05 and fold change ≥ 1.5 was used to identify differences between the P100, EDTA and SST. The EDTA and P100 control plasma tubes were at 0 h post-collection. The SST serum control tube was 30 min post-collection to allow clot formation. The significant metabolites from each comparison were exported to MBRole to determine whether certain chemical classes were more predominantly captured by specific tubes. The top 15 significant chemical taxonomy classes for each comparison, where applicable, is listed numerically (# in set) based on total number of compounds in each class and FDR ≤ 0.05. The complete list is available in Supplemental Document S1 (Supplementary Materials).
| Comparison | Chemical Taxonomy | Category | # in Set | p-Value | FDR |
|---|---|---|---|---|---|
| P100 vs. EDTA | Primary alcohol | HMDB | 17 | 2.63 × 10−4 | 1.21 × 10−2 |
| P100 vs. EDTA | Secondary carboxylic acid amide | HMDB | 11 | 2.04 × 10−4 | 1.21 × 10−2 |
| P100 vs. EDTA | Carboxamide group | HMDB | 11 | 7.48 × 10−4 | 2.29 × 10−2 |
| P100 vs. EDTA | Allyl alcohol | HMDB | 7 | 5.93 × 10−4 | 2.18 × 10−2 |
| P100 vs. EDTA | Amino Acids, Peptides and Analogues | HMDB | 7 | 1.99 × 10−3 | 4.58 × 10−2 |
| P100 vs. EDTA | Fatty Alcohols | HMDB | 5 | 1.21 × 10−4 | 1.11 × 10−2 |
| P100 vs. EDTA | N-acyl-amine | HMDB | 5 | 1.35 × 10−3 | 3.55 × 10−2 |
| P100 vs. EDTA | Lysophosphatidylethanolamines | HMDB | 4 | 1.53 × 10−6 | 2.82 × 10−4 |
| P100 vs. EDTA | Sphingomyelins | Lipid Maps | 3 | 4.31 × 10−5 | 2.11 × 10−3 |
| EDTA vs. SST | Secondary alcohol | HMDB | 44 | 1.68 × 10−4 | 4.75 × 10−3 |
| EDTA vs. SST | Primary alcohol | HMDB | 32 | 3.61 × 10−8 | 1.09 × 10−5 |
| EDTA vs. SST | Glycerophospholipids | Lipid Maps | 24 | 4.36 × 10−4 | 3.23 × 10−2 |
| EDTA vs. SST | 1,2-Diol | HMDB | 21 | 3.54 × 10−3 | 2.97 × 10−2 |
| EDTA vs. SST | Cyclohexane | HMDB | 18 | 1.04 × 10−3 | 1.31 × 10−2 |
| EDTA vs. SST | Secondary carboxylic acid amide | HMDB | 17 | 1.42 × 10−5 | 7.15 × 10−4 |
| EDTA vs. SST | Carboxamide group | HMDB | 17 | 9.65 × 10−5 | 3.24 × 10−3 |
| EDTA vs. SST | Prenol Lipids | HMDB | 17 | 1.61 × 10−3 | 1.58 × 10−2 |
| EDTA vs. SST | Saccharide | HMDB | 16 | 3.11 × 10−3 | 2.68 × 10−2 |
| EDTA vs. SST | Bicyclohexane | HMDB | 11 | 1.31 × 10−3 | 1.41 × 10−2 |
| EDTA vs. SST | Allyl alcohol | HMDB | 10 | 1.73 × 10−4 | 4.75 × 10−3 |
| EDTA vs. SST | Sesterterpene | HMDB | 10 | 1.99 × 10−4 | 5.01 × 10−3 |
| EDTA vs. SST | Decaline | HMDB | 10 | 4.44 × 10−3 | 3.12 × 10−2 |
| EDTA vs. SST | Choline | HMDB | 9 | 2.13 × 10−3 | 1.95 × 10−2 |
| EDTA vs. SST | Quaternary ammonium salt | HMDB | 9 | 4.11 × 10−3 | 3.12 × 10−2 |
| P100 vs. SST | Secondary alcohol | HMDB | 37 | 1.51 × 10−3 | 1.84 × 10−2 |
| P100 vs. SST | Primary alcohol | HMDB | 27 | 1.04 × 10−6 | 3.17 × 10−4 |
| P100 vs. SST | Glycerophospholipids | Lipid Maps | 20 | 1.56 × 10−3 | 2.03 × 10−2 |
| P100 vs. SST | 1,2-Diol | HMDB | 19 | 3.84 × 10−3 | 3.45 × 10−2 |
| P100 vs. SST | Prenol Lipids | HMDB | 17 | 3.49 × 10−4 | 8.42 × 10−3 |
| P100 vs. SST | Cyclohexane | HMDB | 17 | 6.05 × 10−4 | 1.04 × 10−2 |
| P100 vs. SST | Cyclic alcohol | HMDB | 15 | 2.91 × 10−4 | 8.42 × 10−3 |
| P100 vs. SST | Secondary carboxylic acid amide | HMDB | 14 | 1.64 × 10−4 | 7.15 × 10−3 |
| P100 vs. SST | Carboxamide group | HMDB | 14 | 7.69 × 10−4 | 1.12 × 10−2 |
| P100 vs. SST | Saccharide | HMDB | 14 | 5.72 × 10−3 | 4.09 × 10−2 |
| P100 vs. SST | Bicyclohexane | HMDB | 11 | 4.36 × 10−4 | 9.50 × 10−3 |
| P100 vs. SST | Sesterterpene | HMDB | 10 | 6.68 × 10−5 | 5.67 × 10−3 |
| P100 vs. SST | Decaline | HMDB | 10 | 1.71 × 10−3 | 2.01 × 10−2 |
| P100 vs. SST | Drimane-skeleton | HMDB | 8 | 6.15 × 10−4 | 1.04 × 10−2 |
| P100 vs. SST | Allyl alcohol | HMDB | 8 | 1.41 × 10−3 | 1.79 × 10−2 |