Figure 3.

Electrophysiological properties of granule cell neurons (GCNs) are distinctly altered in male and female rats. (a–d) Representative traces of action potentials elicited by depolarizing current injections. Traces were recorded from GCNs from control male (a), methamphetamine male (b), control female (c), and methamphetamine female (d) rats. (e) Legends for bar graphs. (f–l) Comparison of intrinsic and active membrane properties. Analysis across all cells indicated that under baseline conditions resting potential (f) and membrane resistance (g) showed a significant sex × treatment interaction. (h) Graphical relationship between the number of spikes elicited by increasing current injections in current-clamp recording. (i–l) Analysis of electrophysiological properties indicate that AP threshold, rheobase, AHP, and inter-spike interval (ISI) showed significant interaction or main effect of sex or methamphetamine. Data shown are represented as mean ± SEM. Control males, n = 8 cells; control females, n = 6 cells; reinstated males, n = 12 cells; reinstated females, n = 19 cells. %, significant sex × reinstatement interaction; $, main effect of sex; * p < 0.05 vs. control males; * p < 0.05 vs. reinstated males; * p < 0.05 vs. control females by post-hoc tests.