Table 1.

Intracellular (particulate, pg cell⁻¹) and total toxin (particulate + dissolved, pg mL⁻¹cell⁻¹, marked with *) contents in cultures of D. acuta (VGO1065) and D. acuminata (VGO1391) from this work and from other experiments carried out with the same strains isolated in Vigo. Abbreviations: Ref. = reference; ES = M. rubrum strain from Huelva, Spain, or DK = from Denmark, V = volume, T = temperature; sta = stationary phase; exp = exponential phase; L:D = light:dark; OA = okadaic acid; DTX2 = dinophysistoxin 2; PTX2 = pectenotoxin 2.

Species	Ref.	Experimental Conditions	V (mL)	T (°C)	L:D Cycle (h)	Medium	OA (pg cell−1)	DTX2 (pg cell−1)	PTX2 (pg cell−1)
D. acuta	[34]	Well-fed (ES)	250	15	12:12	L1-Si/20	12.2 ± 2.3	4.4 ± 0.9	22.2 ± 9.4
	[34]	Well-fed (ES)	150	17	14:10	L1-Si/20	41.0 ± 4.9	17.4 ± 4.1	38.0 ± 8.2
		Prey-limited (ES)	150	17	14:10		74.1 ± 8.2	32.4 ± 3.8	59.3 ± 11.8
	[34]	Well-fed (DK)	150	17	14:10	L1-Si/20	35.9 ± 7.07	16.5 ± 0.8	70.0 ± 0.8
		Prey-limited (DK)	150	17	14:10		38.6 ± 4.5	19.0 ± 2.3	43.6 ± 6.2
	[33]	Low light	250	15	12:12	L1-Si/40	3.3 ± 1.6	2.2 ± 0.1	71.5 ± 14.2
		High light	250	15	12:12		50.2 ± 20.1	35.4 ± 17.4	187.8 ± 104.1
	This work	Mass culture-sta	1450	15	12:12	L1-Si/20	30.2 *	7.3 *	48.2 *
		Mass culture-sta	3500	15	12:12	K-Si	15.5 *	5.2 *	50.5 *
		Mass culture-exp	4000	19	16:8	K-Si	7.7	2.9	8.2
		Mass culture-sta	5000	15	12:12	L1-Si/20	61.5 *	20.3 *	3 *
D. acuminata	[34]	Well-fed (ES)	250	15	12:12	L1-Si/20	35.2 ± 6.8
	[34]	Well-fed (ES)	150	17	14:10	L1-Si/20	6.0 ± 2.8
		Prey-limited (ES)	150	17	14:10		21.5 ± 0.7
	[34]	Well-fed (DK)	150	17	14:10	L1-Si/20	9.8 ± 1.7
		Prey-limited (DK)	150	17	14:10		32.3 ±4.7
	[33]	Low light	250	15	12:12	L1-Si/40	14.7 ± 12.1
		High light	250	15	12:12		41.4 ± 4
	This work	Mass culture-sta	2200	15	12:12	L1-Si/20	33.3 *
		Mass culture-sta	2700	15	12:12	L1-Si/20	122.2 *
		Mass culture-exp	4000	19	16:8	K-Si	9.9
		Mass culture-sta	4500	15	12:12	K-Si	20.3 *
		Mass culture-sta	17,900	15	12:12	K-Si	28.9 *