Table 1.

Comparison of published deep sequencing–based methods for 2′-O-Me detection.

	RiboMethSeq [53]	2′-OMe-Seq [56]	RibOxi-Seq [57]	(Nm-Seq) [58,59]
Described applications	rRNA, tRNA, (snRNA)	rRNA	rRNA	rRNA, mRNA
RNA input	10 ng (50 ng in routine)	2 × 2 µg	7.5 µg	10 µg rRNA 10 µg polyA mRNA
Sequencing depth	~1000 reads/RNA position (10–15 mln raw reads/sample)	10–15 mln reads/sample	10–15 mln reads/sample	10–15 mln reads/sample
Quantification	Yes	Yes (relative only)	No	No
Sequencing mode used	Single read 50 nt		Paired end 2 × 75 nt

transfer RNAs (tRNAs), ribosomal RNAs (rRNAs), small nuclear RNAs (snRNAs), messenger RNAs (mRNAs).