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. 2018 Dec 17;9(12):638. doi: 10.3390/genes9120638

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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XRN4 is required for proper degradation of AXR3-NT:GUS proteins. Pictures of cotyledons and roots of wt, xrn4-3 and mir393b-1 mutant expressing the HSpro:AXR3-NT:GUS gene. Without treatments, the AXR3-NT:GUS fusion protein is more stable in xrn4-3 and mir393b-1 than in wild-type (wt) plants, and sometimes accumulates ectopically in cotyledons. Clearance of high levels of endogenous AUX/IAA proteins by treatment with 2,4-D for 4 h before heat shock (HS) allowed proper degradation of AXR3-NT:GUS.