Table 4.
Effect of EC supplementation on liver and muscle antioxidant status.
| Characteristic | Vehicle | EC-0.5X | EC-1X | EC-2X | Trend Analysis |
|---|---|---|---|---|---|
| Liver tissues | |||||
| SOD (U/mg) | 2.27 ± 0.10 | 3.01 ± 0.12 ** | 3.09 ± 0.20 ** | 3.25 ± 0.27 *** | 0.0024↑) |
| GPX (nmol/min/mg protein) | 2.04 ± 0.24 | 2.13 ± 0.17 | 2.56 ± 0.24 | 2.88 ± 0.24 * | 0.0048(↑) |
| GR (nmol/min/mg protein) | 1.79 ± 0.12 | 2.07 ± 0.13 | 2.08 ± 0.24 | 2.14 ± 0.24 | 0.2522 |
| CAT (nmol/min/mg protein) | 7.28 ± 0.39 | 9.78 ± 0.31 *** | 10.86 ± 0.33 *** | 12.12 ± 0.32 *** | <0.0001(↑) |
| GSH (µM/mg protein) | 1.57 ± 0.12 a | 1.78 ± 0.13 b | 1.81 ± 0.13 b | 1.81 ± 0.12 b | 0.0015(↑) |
| Muscle tissues | |||||
| SOD (U/mg) | 63 ± 4 | 100 ± 5 *** | 101±6 *** | 105 ± 5 *** | 0.0002(↑) |
| GPX (nmol/min/mg protein) | 201 ± 11 | 206 ± 10 | 206 ± 8 | 207 ± 11 | 0.6676 |
| GR (nmol/min/mg protein) | 122 ± 5 | 122 ± 6 | 126 ± 4 | 129 ± 6 | 0.2710 |
| CAT (nmol/min/mg protein) | 24 ± 1 | 25 ± 1 | 25 ± 1 | 25 ± 2 | 0.6670 |
| GSH (µM/mg protein) | 0.18 ± 0.01 | 0.19 ± 0.02 | 0.19 ± 0.02 | 0.19 ± 0.02 | 0.8085 |
Mice were supplemented with the vehicle, EC-0.5X, EC-1X and EC-2X for 28 days. Data is expressed as mean ± SEM (n = 10). The up arrow (↑) indicates EC dose-dependent increase. Significant difference from control group according to one-way ANOVA is indicated * p < 0.05, ** p < 0.01, *** p < 0.001.