Table 2.
Primer | Sequence |
---|---|
Adapters | |
EcoRI adapter | 5’-CTCGTAGACTGCGTACC-3’ |
5’-AATTGGTACGCAGTCTAC-3’ | |
MseI adapter | 5’-GACGTGAGTCCTGAG-3’ |
5’-TACTCAGGACTCAT-3’ | |
Pre-amplification primer | |
EcoRI | 5’-GACTGCGTACCAATTC-3’ |
MseI | 5’-GATGAGTCCTGAGTAA-3’ |
Selective amplification primer | |
E-AAC/M-CTC | 5’-GACTGCGTACCAATTCAAC-3’ 5’-GATGAGTCCTGAGTAACTC-3’ |
E-AAG/M-CAA | 5’-GACTGCGTACCAATTCAAG-3’ 5’-GATGAGTCCTGAGTAACAA-3’ |
E-AAG/M-CAC | 5’-GACTGCGTACCAATTCAAG-3’ 5’-GATGAGTCCTGAGTAACAC-3’ |
E-AGC/M-CAC | 5’-GACTGCGTACCAATTCAGC-3’ 5’-GATGAGTCCTGAGTAACAC-3’ |