REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
SUMO2/3 mouse monoclonal	Abcam	Cat# ab81371; RRID:AB_1658424
SUMO2/3 mouse monoclonal	University of Iowa	8A2: RRID: AB_2198421
HSP40 rabbit polyclonal	Enzo	Cat# ADI-SPA-400; RRID:AB_10631418
HSP70 rabbit polyclonal	Enzo	Cat# ADI-SPA-811D; RRID:AB_2621431
β-Actin mouse monoclonal	Sigma	Cat# A1978; RRID:AB_476692
ACF1/Baz1A rabbit polyclonal	Bethyl Laboratories	Cat# A301-318A-T; RRID:AB_937730
RCH1/KPNA2 rabbit polyclonal	Bethyl Laboratories	Cat# A300-483A-T; RRID:AB_451018
MMS21 rabbit polyclonal	Bethyl Laboratories	Cat# A300-129A; RRID:AB_2142840
NPM 1 rabbit polyclonal	Bethyl Laboratories	Cat# A302-402A; RRID:AB_1907285
SENP1 rabbit monoclonal	Abcam	Cat# ab108981; RRID:AB_10862449
SENP3 rabbit monoclonal	Cell Signaling Techology	Cat# 5591P; RRID:AB_10695540
SETDB1 rabbit monoclonal	Cell Signaling Techology	Cat# 2196S; RRID:AB_823637
SENP6 mouse monoclonal	Sigma	Cat # WHP0026054M1; RRID:AB_1843525
FoxM1	Santa Cruz	Cat# sc-502; RRID:AB_631523
Ubiquitin Mouse monoclonal	Millipore	Cat# 04-263; RRID:AB_612093
Ubiquitin	Santa Cruz	Cat# sc-8017; RRID:AB_628423
Bacterial and Virus Strains
BL21(DE3)	New England BioLabs	Cat# C2527I
Chemicals, Peptides, and Recombinant Proteins
STA-9090	MedChem Express	Cat# HY-15205
Doxycyline	Alfa Aesar	Cat# J63805
Trimethoprim	Matrix	Cat# 058373
Bortezomib	ChemGood	Cat# C-1279
MG-132	Fischer Scientific	Cat# NC9326288
Resazurin Sodium Salt	Sigma	Cat# R7017-5G
Rho-SUMO2-PA	(Mulder et al., 2018)	N/A
K11 di-SUMO VA ABP	(Mulder et al., 2018)	N/A
Critical Commercial Assays
4–12% Bolt™ Bis–Tris	ThermoFisher	NW04125BOX
NuPage™ 3–8% Tris-acetate	ThermoFisher	EA03755BOX
EZNA Total RNA Kit I	Omega	Cat# R6834-02
High-Capacity cDNA Reverse Transcription Kit	Applied Biosystems	Cat# 4368814
Fast Start Universal SYBR Green Master Mix	Roche	Cat# 4913850001
Deposited Data
ProteomeXchange (via PRIDE database)	This paper	PXD010231
Experimental Models: Cell Lines
Human: HEK293T-REx	Life Technologies	Cat# R71007
Human: LX2	EMD Millipore	Cat# SCC064; RRID:CVCL_5792
Recombinant DNA
pET-15b	Novagen	Cat# 69661
pET11a-SUMO3	(Flotho et al., 2012)	Addgene Plasmid #53143
pDEST17	Invitrogen	Cat# 11803012
pT-E1E2S2	(Uchimura et al., 2004)	N/A
Lentiviral His10-SUMO2 IRES GFP	(Hendriks and Vertegaal, 2016)	N/A
Lentiviral His10-Ubiquitin IRES Puro	(Cuijpers et al., 2017)	N/A
pLenti CMV/TO Zeo dn-cHSF1	(Moore et al., 2016)	N/A
pLenti CMV/TO Zeo GFP	(Moore et al., 2016)	N/A
pLenti CMV Puro DHFR.dn-cHSF1	(Moore et al., 2016)	N/A
Software and Algorithms
MaxQuant ver 1.5.6.0	Max Planck Institute	http://www.biochem.mpg.de/5111795/maxquant
Perseus Software ver. 1.5.6	Max Planck Institute	http://www.biochem.mpg.de/5111810/perseus
PANTHER	Gene Ontology Consortium	http://pantherdb.org/
Image Studio Lite ver 5.2	Li-COR	https://www.licor.com/bio/products/software/
GraphPad Prism 7	GraphPad	https://www.graphpad.com
ImageJ	Fiji	https://imagej.net/Welcome
Other
Triple-disc C18 reverse phase stage-tips	(Rappsilber et al., 2007)	N/A