Figure 6.

Reactive oxygen species production and lipid peroxidation in human, mouse, and hamster RPTECs under anoxia or reoxygenation. Anoxia did not alter ROS in any of the evaluated cells, whereas reoxygenation increased ROS in all of them (A). Lipid peroxidation, a known mechanism of cell death, was assessed by the level of cellular malondialdehyde (MDA). Anoxia did not affect MDA in any of the evaluated cells. Reoxygenation induced a dramatic increase in MDA level in human and mouse RPTECs, but it did not alter the MDA level in hamster RPTECs (B). Hu: human; Mo: mouse; and Hm: hamster. Asterisk indicates a p < 0.05 compared to the control cultured under normoxia cells, and error bars correspond to SD.