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. 2018 Dec 12;10(12):707. doi: 10.3390/v10120707

Figure 1.

Figure 1

(A) Agarose gel electrophoresis of the RT-PCR product showing a ~3 kb nested PCR amplicon that was confirmed by Sanger sequencing as cDNA amplicon of RirMV1. Left lane: 1 kb ladder. Right lane: RirMV1 amplicon of the predicted size of 3 kb and (B) Agarose gel electrophoresis of the RT-PCR product showing no amplification, suggesting the viral contig of RNA-Seq was not originated from Medicago root without R. irregularis strain 09 infection. Left to right lanes: 1 kb ladder, viral primers, plant primers.