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Subculture of B. burgdorferi after treatment with essential oils. A B. burgdorferi stationary phase culture (seven-day old) was treated with the indicated essential oils at 0.1% (A) or 0.05% (B) for seven days followed by washing and resuspension in fresh BSK-H medium and subculture for 21 days. The viability of the subculture was examined by SYBR Green I/PI stain and fluorescence microscopy.
