Table 2.
Comparison of top 10 essential oil activities against stationary phase B. burgdorferi with 0.1% and 0.05% (v/v) treatment and subculture a.
| Essential Oil Treatment | Residual Viability after 0.1% Essential Oil Treatment | Residual Viability after 0.05% Essential Oil Treatment | ||
|---|---|---|---|---|
| Treatment b | Subculture c | Treatment b | Subculture c | |
| Drug free control | 93% | + | 93% | + |
| Daptomycin+Doxycycline+Cefuroxime d | 18%d | − d | N/A | N/A |
| Garlic | 30% | − | 33% | − |
| Allspice | 34% | − | 48% | + |
| Myrrh | 42% | − | 41% | + |
| Hydacheim | 44% | − | 61% | + |
| Litsea cubeba | 68% | − | 69% | + |
| Palmarosa | 39% | + | 67% | + |
| Lemon eucalyptus | 46% | + | 79% | + |
| Amyris | 48% | + | 71% | + |
| Cumin | 42% | + | 60% | + |
| Thyme white | 40% | + | 76% | + |
| Cinnamaldehyde | 34% | − | 56% | − |
a A 7-day old stationary phase B. burgdorferi was treated with 0.05% or 0.1% essential oils for seven days when the viability of the residual organisms was assessed by subculture. b Residual viable percentage of B. burgdorferi was calculated according to the regression equation and ratio of Green/Red fluorescence obtained by SYBR Green I/PI assay as described [22]. Viabilities are the average of three replicates. c “+” indicates growth in subculture; “−” indicates no growth in subculture. d Activity was tested with 5 μg/mL of each antibiotic in combination.