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. 2018 Dec 17;10(12):521. doi: 10.3390/cancers10120521

Figure 7.

Figure 7

AKNA restoration induces expression of IL-8 in HPV positive cell lines. HeLa cells were transfected with pcDNA3-akna plasmid and after 24 h, total RNA was extracted using Trizol following the manufacturer’s instructions. Complementary DNA was synthesized and used as a template for IL-8 mRNA amplification using standard PCR (A) or relative quantification (B). AKNA transfected cells showed a remarkable increased in the IL-8 mRNA levels after AKNA expression. Data is presented as relative expression of IL-8 after normalizing with the HPRT1 expression ± SD. * p = 0.017 vs. pcDNA3 control. n = 4.