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. 2018 Dec 7;9(12):612. doi: 10.3390/genes9120612

Figure 3.

Figure 3

Evaluating mechanisms of decreased function of human tRNASerUGG in yeast. (A) BY4742 (MET22) and CY7641 (met22Δ) containing either wild-type (WT) SctRNASerUGA or HstRNASerUGG were grown to stationary phase in media lacking uracil before cells were spotted in 10-fold serial dilutions onto a plate lacking uracil and grown at 30°. (B) Expression of human SerRS, SARS, increases the functionality and thus the toxicity of HstRNASerUGG and chtRNASerUGG in yeast. Yeast strain BY4742 containing a URA3 centromeric plasmid expressing SctRNASerUGA, SctRNASerUGG-G26A. HstRNASerUGG or chtRNASerUGG and the LEU2 multicopy plasmid YCplac181 containing myc-tagged yeast SES1 (ySerRS) or human SARS (hSerRS) were grown to stationary phase in media lacking uracil and leucine. Cell densities were normalized, and cultures were spotted in 10-fold serial dilutions on the same media.