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. 2018 Dec 7;9(12):612. doi: 10.3390/genes9120612

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The acceptor stem 3:70 base pair is a determinant of yeast versus human tRNA^Ser specificity. (A) Variation in the acceptor stem of the S. cerevisiae tRNA^Ser isoacceptors. The acceptor stem of the tRNA encoded by SUP17 is shown with the positions that vary in the different cytoplasmic tRNA^Ser isoacceptors colored in red. (B) Variation in the acceptor stems of tRNA^Ser_UGA between S. cerevisiae and humans. The acceptor stem of the tRNA encoded by SUP17 is shown with the bases always different between yeast and human colored in red and those sometimes-different colored in blue. (C) BY4742 expressing chtRNA^Ser_UGG or chtRNA^Ser_UGG-C3:G70 on a URA3 centromeric plasmid were transformed into BY4742 and grown at 30 °C for 2 days.