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. 2018 Nov 23;16(12):465. doi: 10.3390/md16120465

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Characterization and purification of C. reniformis marine collagen hydrolysates (MCHs). (A) SDS-PAGE analysis of undigested and digested sponge collagen. C. reniformis trypsin-digested collagen solutions, two different preparations (lane 1 and 2), and undigested collagen suspension (lane 3) were analyzed on 7.5% SDS polyacrylamide gel and Coomassie blue stained. std = standard molecular weight markers. In lane 3, highlighted in the box, α1-chain and α2-chain of fibrillar collagen. (B) RP-HPLC (reversed phase high-performance liquid chromatography) profile of the C. reniformis trypsin-digested collagen solution in the chromatography used to obtain the MCH fractions. During the purification, fractions were collected every two minutes, as indicated by the vertical dotted grey lines on the chromatogram. The fractions of interest are indicated by the abbreviations M3, M4, M5, and M6, respectively. The analytical conditions are reported in the Materials and Methods (Section 4.4). The continuous line indicates the chromatogram registered at 220 nm, while the dotted line indicates the chromatogram of the same run at 254 nm.