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. 2018 Nov 1;5(4):95. doi: 10.3390/bioengineering5040095

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Characteristics of MSCs derived from non-vitrified and vitrified WJ tissue samples. (A) Differentiation potential of MSCs from non-vitrified (n = 3) WJ tissue samples towards “osteogenic (1)”, “adipogenic (3)” and “chondrogenic (5)” lineages. Differentiation potential of MSCs from vitrified (n = 3) WJ tissue samples towards “osteogenic (2)”, “adipogenic (4)” and “chondrogenic (6)” lineages. Original magnification 10×, scale bars 100 μm (B) CFU-F assay of WJ-MSCS obtained from non-vitrified (n = 3) and vitrified (n = 3) WJ tissue samples. (C) Counting of CFUs. Total cell number (D), CDT (E), PD (F), % cell viability (G) of MSCs until reaching passage 8 from non-vitrified (n = 3) and vitrified (n = 3) WJ tissue samples.