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. 2018 Nov 22;10(12):464. doi: 10.3390/cancers10120464

Figure 3.

Figure 3

Endogenous ISG15 expression suppresses ovarian cancer growth and induces apoptosis in vitro. (A) Endogenous ISG15 expression levels were evaluated in eight ovarian cancer cell lines by Western bolting analysis. ISG15 expression in OVCA432 ovarian cancer cells was silenced by transduction of ISG15 specific shRNAs. ISG15 silencing was confirmed by (B) qRT-PCR and (C) Western blot analyses. (D) OVCA432 cells transduced with ISG15 shRNAs showed a significant increase in growth rates compared with those transduced with the control construct (p < 0.001). (E) Cell Death ELISA assay demonstrated a significant decrease in apoptosis in OVCA432 cells transduced with ISG15 shRNAs compared with the scramble sequence. (F) ALST ovarian cancer cells were transduced with a full-length ISG15 expression construct. Increased ISG15 protein expression in transduced cells were confirmed by Western blot analysis. In vitro experimental results showed a significant (G) decrease in growth rate and (H) increase in apoptosis in cells transduced with the ISG15 expression vector when compared with those transduced with the control vector.