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Relative genome copies-to-TCID50 ratio of reporter IAVs PR8-NS1-Gluc and PR8-PB2-Gluc. Indicated viruses derived from MDCK were titrated and extracted for vRNAs followed by quantification of segment M by qPCR. The genome copies-to-TCID50 ratio were evaluated. The standard deviations were calculated based on three replicates. * p < 0.05; ns, no significance, students’ t test.
