Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Nov 30;10(12):679. doi: 10.3390/v10120679

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

vRNA NS-Gluc is less competitive than natural NS in replication. RdRp assays were performed by cotransfecting pPolI-NS and pPolI-NS-Gluc respectively with pDZ-M as well as expression plasmids PA, PB1, PB2 and NP. At 30 h post transfection, the cells were harvested and total RNA were extracted. (a) The concentrations of vRNA NS and NS-Gluc were normalized to vRNA M, and the relative concentrations were presented; (b) The concentrations of mRNA NS, NS-Gluc and M were normalized to mRNA NP, and the relative concentrations were shown. The standard deviations were calculated based on three replicates. *** p < 0.001; * p < 0.05; students’ t test.