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The replication of vRNA NS-Gluc and PB2-Gluc were impaired in infected cells. (a–h) Replication kinetics of indicated vRNAs in MDCK cells infected with PR8, PR8-NS1-Gluc, PR8-PB2-Gluc, respectively; (i,j) The relative concentrations of vRNAs in MDCK cells infected with indicated reporter IAVs were presented as the ratios of each vRNA segment versus M. The standard deviations were calculated based on three replicates. *** p < 0.001, students’ t test.
