Figure 2.

Pro-1 in p6 impairs IDE-mediated degradation. Lysates of VLPs derived from pNLenv1 wt and L1P (A) or viral particles of SG3 wt and P1L (B) were incubated with S10. Reactions were stopped by heat inactivation either immediately or after incubation for 60 min at 37 °C. Representative Western blots of three independent experiments are shown. vp6 derived from NL4-3 wt and L1P (C) or SG3 wt and P1L (D) were incubated with S10 for the times indicated. Samples were analyzed by Western blotting. (E) Time kinetics of three independently performed experiments are shown. Values represent the arithmetic mean ± SD.