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. 2018 Dec 7;7(12):256. doi: 10.3390/cells7120256

Figure 3.

Figure 3

Role of p38 MAPK/CREB and GSK3β/CREB axis in regulating IL-10 and IL-12 in GM/DCs. Bone marrow-derived GM/DCs were preincubated for 1 h in the presence of a p38 MAPK (SB203580, 10 µM) or a GSK3 (SB216763, 10 µM) inhibitor or, vehicle (DMSO) before being exposed to LPS (1 μg/mL for 4 h or 12 h). Total RNA was isolated and the expression of IL-10 (A), IL-12p35 (B) and IL-12p40 (C) was determined by real-time PCR. Fold increases are calculated with reference to vehicle (DMSO) + LPS conditions. Data are representative of five or six independent experiments. In the case of cytokine release essays, GM/DCs were preincubated for 1 h with SB203580 (10 µM), SB216763 (10 µM) or, with vehicle (DMSO), before being exposed to LPS (1 μg/mL for 24 h). IL-10 (D) and IL-12p70 (E) production was quantified in cell supernatants by ELISA. Data are representative of three independent experiments. Results are shown as the mean ± SEM. The asterisks (*) correspond to p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***), one-way ANOVA with post hoc Bonferroni’s test.