Effect of C/EBPβ deficiency on the maturation status of GM/DCs. Bone marrow-derived GM/DCs generated from C/EBPβ+/+ mice (Blue line) and C/EBPβ−/− mice (red line), were left unstimulated (A) or exposed to LPS (1 µg/mL for 48 h) (C) and were stained and analyzed for CD80, CD86, and CD40 expression on CD11c+ cells using flow cytometry. Dashed thin lines represent labeling with isotype control antibodies. (B and D) Expression levels of CD80, CD86, and CD40 cell surface markers on unstimulated (B) and LPS exposed (D) GM/DCs are illustrated as mean fluorescence intensities (MFIs). The results are representative of three independent experiments. * p < 0.05, nonparametric unpaired Mann—Whitney test.