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. 2018 Dec 7;7(12):256. doi: 10.3390/cells7120256

Figure 6.

Figure 6

Effect of C/EBPβ deficiency on gene expression and production of IL-10 and IL-12 by GM/DCs. Bone marrow-derived GM/DCs generated from C/EBPβ−/− or C/EBPβ+/+ mice were left unstimulated or were stimulated with 1 µg/mL of LPS for 4 h and 12 h. The expression of IL-10 (A), IL12p35 (B), IL-12p40 (C), and IL-6 (D) was determined by real-time PCR. Data are shown as fold increases with reference to unstimulated GM/DCs of C/EBPβ+/+ (t = 0) and are representative of four independent experiments. For cytokines release, bone marrow-derived GM/DCs generated from C/EBPβ−/− or C/EBPβ+/+ mice were left unstimulated or stimulated with LPS (1 µg/mL for 24 h). Cell supernatants were collected and IL-10 (E), IL-12p70 (F), and IL-6 (G) production was analyzed by ELISA. Data are representative of three independent experiments and are shown as the mean ± SEM. The asterisks (*) correspond to p < 0.01 (**) and p < 0.001 (***), one-way ANOVA with post hoc Bonferroni’s test.