Figure 8.
Effect of reducing treatments on AtMDHAR6 activity. A sample of purified recombinant AtMDHAR6 enzyme was incubated for 15 min at room temperature prior to measuring its NADPH-dependent activity. Incubation was in absence of DTT (white bar), or in presence of DTT, alone (blue bar), or with TRX: f1 (green bar), or m1 (yellow bar), or x (purple bar), or y2 (red bar). Data correspond to means ± SD (n = 4, from 2 independent experiments). The treatment significantly increasing NADPH-MDHAR activity (comparison with untreated) (p < 0.05) is indicated by an asterisk (*).