Figure 1.

(a) SPR screening assay for crude antibodies. The first image shows the capture of a crude anti-hazelnut mAb (blue) via its FC region by the FC specific IgG (orange). The second image shows the binding of total hazelnut protein to the anti-hazelnut mAb. The third image displays the sandwich pairing of an anti-hazelnut mAb (blue) towards hazelnut and another anti-hazelnut mAb (blue). (b) Normalised SPR sensorgrams for 12 crude antibody preparations against 20 ppm hazelnut extract. The hazelnut injection is indicated by the first arrow, which is followed by the association of the crude antibodies towards hazelnut. The second arrow indicates the start of the hazelnut dissociation from the antibodies. (c) Full sensorgram of a crude antibody towards hazelnut in triplicate. The first curve shows the capture of the crude antibody via its FC region, the second curve the binding of hazelnut to that antibody and the following two spikes the regeneration.