Figure 4.

Effect of ligands on the CFA-induced thermal hyperalgesia test. Test was performed 2 h after intramuscular administration of ASIC3 antagonists. (A) Comparison between ligands at the dose of 1 mg/kg; (B–D) Dose-dependent chart of ligands’ effect. APETx2 (B), sevanol (C), and Ugr9-1 (D) reversed CFA-induced thermal hyperalgesia and prolonging withdrawal latency of the inflamed hind paw on a hot plate. Results are presented as mean ± SEM (n = 7–8). * p < 0.05, ** p < 0.01, *** p < 0.001 versus saline group (ANOVA followed by a Tukey’s test).