Figure 5.

AZC elevates LC3-II levels in a Ca²⁺-dependent manner. Cells were treated for 6 h with 5 mM AZC in absence or presence of 10 μM or 20 μM of the intracellular Ca²⁺ chelator BAPTA-AM, and during the last 4 h 100 nM Baf A1 was added. (A) A representative blot of six independent experiments each performed in duplicate, assessing protein levels of the autophagy marker LC3-II. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as loading control. (B) Quantification of LC3-II levels in (A) shown as mean ± SEM relative to untreated control. * p < 0.05.