Figure 1.

Impact of T-VDA^CV venom array on MetHb production. Whole snake venoms (0.2 mg/mL) from the T-VDA^CV array (Venomtech Limited, UK) targeting the cardiovascular system were dispensed into 96-well micro titre plates, mixed with an equal volume of ovine Hb, and incubated at 37 °C for 16 h. The absorbance was measured at 500 nm and 630 nm (i.e., the peaks typically observed for MetHb) by spectrofluorimetry (FLUOstar Optima, BMG Labtech) at 16 h. Data represent mean ± SEM (n = 3), and the p values (all the values were compared to the negative control [PBS]) were calculated by One-way ANOVA followed by post-hoc Tukey’s test using GraphPad Prism (* p < 0.05, ** p < 0.01, and **** p < 0.0001).