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. 2018 Dec 13;8(4):173. doi: 10.3390/biom8040173

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The MALDI-TOF mass spectra (positive ionization mode) of 1,2-dipalmitoyl-sn-phosphatidylethanolamine (PE 16:0/16:0; M = 691.5 g/mol), after treatment with a ten-fold molar excess of hypochlorous acid (HOCl) leading to the dichloramine of PE (Cl₂PE) 16:0/16:0 using the following matrices: (a) DHB; (b) α-cyano-4-hydroxycinnamic acid (HCCA), and (c) 4-chloro-α-cyanocinnamic acid (ClCCA). Please note the exclusive detectability of the Cl₂PE 16:0/16:0 as singly- and doubly-sodiated adducts at m/z 782.5 and 804.5, respectively, in the case of ClCCA (c). In all other cases, only PE 16:0/16:0 could be detected as protonated (m/z 692.5), sodiated (m/z 714.5), and as [(PE 16:0/16:0) − H + 2Na]⁺ at m/z 736.5. Reprinted with permission from Elsevier (with modifications) [104].