Table 5.

Primers and locus specific annealing temperatures (Ta) for PCR amplifications.

Primer Pair	Target Gene	Sequence	Ta (°C)	Reference
Bt2a-2b	β-tubulin	F-GGTAACCAAATCCGTGCTGCTTTC	51	[50]
		R-ACCCTCAGTGTAGTGACCCTTGGC
Bt3a-3b		F-CGTCGTTCATTCGAGGTGTA	56	Current study
		R-CCGCTCAACTTCAAGTCCAT
cmd42-637	Calmodulin	F-GGCCTTCTCCCTATTCGTAA	56	[16]
		R-CTCGCGGATCATCTCATC
cmd2F-2R		F-GGCTGGATGTGTGTAAATC	48	[16]
		R-ATTGGTCGCATTTGAAGGG
niaDF-AR	Nitrate reductase	F-CGGACGATAAGCAACAACAC	52	[16]
		R-GGATGAACACCCGTTAATCTGA
niaDBF-BR		F-ACGGCCGACAGAAGTGCTGA	57	[16]
		R-TGGGCGAAGAGACTCCCCGT
niaDCF-CR		F-GCAGCCCAATGGTCACTACGGC	55	[12]
		R-GGCTGCACGCCCAATGCTTC