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. 2018 Nov 30;10(12):479. doi: 10.3390/cancers10120479

Figure 2.

Figure 2

Human AGS cells with HP infection expresses high HDGF level. HDGF mRNA in AGS cells with HP49503 infection for 24 h was analyzed by RT-q-PCR (A). HDGF protein from AGS cells infected with HP49503 for 24 h was detected by immunoblotting assay. GAPDH was used as an internal control (B). Immunofluorescent staining was used to detect the expression of HDGF in AGS cells with HP49503 infection. Scale bar = 20 μm. (C). Secreted HDGF from AGS cells with HP49503 infection was measured by ELISA assay (D). * p < 0.05 versus control (mean ± SD, n = 3).