Table 1.
Markers for myofibroblasts and fibroblasts.
| Primer | Sequence (5′ to 3′) | Orientation | |
|---|---|---|---|
| Myofibroblast | human α smooth muscle actin (α-SMA) | ACCCACAATGTCCCCATCTA | Forward |
| human α smooth muscle actin (α-SMA) | GAAGGAATAGCCACGCTCAG | Reverse | |
| human procollagen α-I | GTGCTAAAGGTGCCAATGGT | Forward | |
| human procollagen α-I | ACCAGGTTCACCGCTGTTAC | Reverse | |
| human tropomyosin 1 | GAAGCTCGACAAGGAGAACG | Forward | |
| human tropomyosin 1 | TTTTGCAGTGACACCAGCTC | Reverse | |
| human desmin | CAAGCTGCAGGAGGAGATTC | Forward | |
| human desmin | GAGATTCAATTCTGCGCTCC | Reverse | |
| human fibroblast activation protein α (FAP-α) | CGATACCACTTACCCTGCGT | Forward | |
| human fibroblast activation protein α (FAP-α) | ATCAGTAACCCACGTGAGCC | Reverse | |
| Fibroblast | human prolyl 4-hydroxylase A1 (PHA1) | GGCAGCCAAAGCTCTGTTAC | Forward |
| human prolyl 4-hydroxylase A1 (PHA1) | AAAGCAGTCCTCAGCCGTTA | Reverse | |
| human fibroblast specific protein-1 (FSP-1) | GATGAGCAACTTGGACAGCA | Forward | |
| human fibroblast specific protein-1 (FSP-1) | CTTCCTGGGCTGCTTATCTG | Reverse | |
Oligonucleotide primers used for q-PCR.