Figure 6.

α-Syn shRNA promoted KCl-evoked synaptic vesicle fusion during Mn exposure. After treatment with different doses of Mn, cells were incubated with 100 μM FM1-43 for 2 min in HBM containing 1mM Ca²⁺ and 30 mM K⁺. (A) The FM1-43 fluorescence before KCl-evoked exocytosis. (B) The FM1-43 fluorescence 30 min after 15 mM KCl-evoked exocytosis. Untreated sample represented the normal downloading of the fluorescent dye in neurons neither KCl-evoked nor Mn-treated. (C) Fluorescence intensity before and after KCl-evoked exocytosis in different groups normalized with those of controls. (D) Relative fluorescence intensity between (A) and (B) in different groups normalized with those of controls. Data represent mean ± standard deviation, n = 4. * p < 0.05 and ** p < 0.01 indicates significant differences from controls; ^# p < 0.05 indicates significant differences from the 100 μΜ Mn-treated group. Scale bars: 50 μm.