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. 2018 Dec 8;7(12):260. doi: 10.3390/cells7120260

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Von Kossa staining of cells at day 16 of osteogenic differentiation; LDSCs in osteogenic medium (OS) (a) LDSCs in standard medium (DM) (b) ADSCs in OS medium (e) and ADSCs in DM medium (f); dark grey/black deposits’ color in (a) and (e) indicates positive staining; Alizarin red S staining of cells at day 16 of osteogenic differentiation; LDSCs in OS medium (c) LDSCs in DM medium (d) ADSCs in OS medium (g) and ADSCs in DM medium (h); red deposits’ color in (c) and (g) indicates positive Alizarin red S (ARS)staining; All images were acquired at bright field with objective magnification 10×; quantitative measurement of ARS dye (i), presented as mean ± SD, n (LDSCs) = 5 and n (ADSCs) = 4 for all groups; (*) p < 0.05.