Table 1.
Antibodies included in the T‐cell and myeloid panels
| Target | Clone | Species | Isotype | Detection | Fluorochrome | Supplier |
|---|---|---|---|---|---|---|
| T‐cell panel | ||||||
| Pan‐Cytokeratin | AE1/AE3 and C11 | Mouse | IgG1 | Direct | Alexa 488 | Thermo Fisher Scientific |
| Cell Signaling Technology | ||||||
| CD8 | 4B11 | Mouse | IgG2b | Indirect | CF555 | DAKO |
| CD3 | D7A6E | Rabbit | IgG | Direct | Alexa 594 | Cell Signaling Technology |
| FOXP3 | 236A/E7 | Mouse | IgG1 | Indirect | CF633 | Thermo Fisher Scientific |
| CD45RO | UCHL1 | Mouse | IgG2a | Indirect | CF647 | Cell Signaling Technology |
| GZMB | EPR8260 | Rabbit | IgG | Indirect | Alexa 680 | Abcam |
| DAPI | ||||||
| Myeloid panel | ||||||
| PDL1 | SP‐142 | Rabbit | IgG | Opal | Opal 520 | Spring Bioscience |
| CD11c | EP1347Y | Rabbit | IgG | Direct | Alexa 546 | Abcam |
| HLA‐DR | TAL 1B5 | Mouse | IgG1 | Direct | Alexa 594 | Thermo Fisher Scientific |
| CD163 | 10D6 | Mouse | IgG1 | Indirect | CF633 | Thermo Fisher Scientific |
| Pan‐Cytokeratin | AE1/AE3 and C11 | Mouse | IgG1 | Direct | Alexa 647 | Novus Biologicals/Biolegend |
| CD68 | D4B9C | Rabbit | IgG | Indirect | Alexa 680 | Cell Signaling Technology |
| DAPI | ||||||