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. Author manuscript; available in PMC: 2019 Jan 3.
Published in final edited form as: Methods. 2015 Sep 1;96:46–58. doi: 10.1016/j.ymeth.2015.08.024

Fig. 2.

Fig. 2.

High-content imaging quantification of HGPS defects. (A) Schematic representation of the pipeline used for automated detection and quantification of HGPS defects in GFP-progerin inducible fibroblasts. (B, C, E) Representative image of nuclear segmentation based on the DNA DAPI signal in GFP-progerin inducible fibroblasts (4 days, 1 μg/ml doxycycline), shown for DAPI stain (A), GFP-progerin (B) and lamin B1 IF staining (D); scale bar: 10 μM. (D) Example of contrast-based segmentation of γH2AX foci in GFP-progerin inducible fibroblasts (4 days, 1 μg/ml doxycycline). Scale bar: 10 μM.