. 2018 Dec 20;2018:4989602. doi: 10.1155/2018/4989602

Table 2.

Primers used in the study for the detection of β-lactamase-encoding genes.

Primer	Target(s)	Sequence (5′-3′)	Annealing temperature (°C)	Amplicon size (bp)	Reference
veb-sf	bla _VEB	GATTGCTTTAGCCGTTTTGTC	50	452	[15]
veb-sr	bla _VEB	ATCGGTTACTTCCTGTTGTTGTTTC	50	452	[15]
z-sf	bla _Z	ACAGTTCACATGCCAAAGAGT	50	479	[16]
z-sr	bla _Z	CTTACCGAAAGCAGCAGGTG	50	479	[16]
mir-sf	bla _MIR	GCCGCACCGATGTCCGAAAAA	50	545	[18]
mir-sr	bla _MIR	GGTTTAAAGACCCGCGTCGTCATGG	50	545	[18]
azecl-29-sf	bla _AZECL-29	GTCTTTACGCTAACACCAGCATCGG	50	381	[17]
azecl-29-sr	bla _AZECL-29	TCAGCATTTCCCAGCCCAATC	50	381	[17]
veb-ff	bla _VEB	CGGGATCCATGAAAATCGTAAAAAGGATATTAT	50	918	This study
veb-fr	bla _VEB	CCCAAGCTTTATTTATTCAAATAGTAATTCCACG	50	918	This study
z-ff	bla _Z	CGGGATCCATGAAAAAGTTAATATTTTTAATTG	50	864	This study
z-fr	bla _Z	CCCAAGCTTTAAAATTCCTTCATTACACTCTTG	50	864	This study
mir-ff	bla _MIR	CGGGATCCATGATGACAAAATCCCTAAGCTGTG	66	1164	This study
mir-fr	bla _MIR	CCCAAGCTTTACTGCAGCGCGTCGAGGATACGG	66	1164	This study
azecl-29-ff	bla _AZECL-29	CGGAATTCATGATGAAAAAAAACCTAAGCTGTG	60	1164	This study
azecl-29-fr	bla _AZECL-29	CCCAAGCTTTACTGCAGCGCGTCGAGGATACG	60	1164	This study
ctx-m-9a-ff	bla _CTX-M-9a	CGGGATCCATGGTGACAAAGAGAGTGCAACGGA	60	876	This study
ctx-m-9a-fr	bla _CTX-M-9a	CCAAGCTTTTACAGCCCTTCGGCGATGATTCTC	60	876	This study
shv-12-ff	bla _SHV-12	CGGGATCCGTGGTTATGCGTTATATTCGCCTGT	60	867	This study
shv-12-fr	bla _SHV-12	CCAAGCTTTTAGCGTTGCCAGTGCTCGATCAGC	60	867	This study

Underlined sequences denote restriction endonuclease sites. sf: forward screening primer; sr: reverse screening primer; ff: forward full-length primer; fr: reverse full-length primer.