Table 1.
Oligonucleotide primers and amplicon length of PCR products.
| Gene information | Primer sequence | Amplicon length | Accession number | Reference |
|---|---|---|---|---|
| IL1B1 (interleukin-1, beta 1, Sus scrofa) | (S) 5′- CCT CCT CCC AGG CCT TCT GT -3′ (AS) 5′- GGG CCA GCC AGCA CTA GAG A -3′ |
178 bp | [31] | |
| IL-18 (interleukin-18, Sus scrofa) | (S) 5′- ACG ATG AAG ACC TGG AAT CG -3′ (AS) 5′- GCC AGA CCT CTA GTG AGG CTA -3′ |
205 bp | AF191088.1 | |
| NLRP3 (NLR family, pyrin domain containing 3, Sus scrofa) | (S) 5′- AGC AGA TTC CAG TGC ATC AAA G -3′ (AS) 5′- CCT GGT GAA GCG TTT GTT GAG-3′ |
75 bp | NM_001256770.2 | [32] |
| NLRC4 (NLR family, CARD domain containing 4, Sus scrofa) | (S) 5′- TGC TCT GAA ACA CCT TGC AT -3′ (AS) 5′- GCA TAG ATT CCT GCC TCC AG -3′ |
92 bp | XM_013987922.1 | |
| CASP13 (caspase-13, apoptosis-related cysteine peptidase, Sus scrofa) | (S) 5′- GTG CTA CAG AAA CGC CAT GA -3′ (AS) 5′- AGG GCA AAG CTT GAG GGT AT-3′ |
150 bp | XM_003129812.6 | |
| CASP1 (caspase-1, apoptosis-related cysteine peptidase, Sus scrofa) | (S) 5′- CTC TCC ACA GGT TCA CAA TC -3′ (AS) 5′- GAA GAC GCA GGC TTA ACT GG -3′ |
116 bp | NM_214162 | [33] |
| ASC (LOC100522011) (apoptosis-associated speck-like protein containing a CARD, Sus scrofa) | (S) 5′- CCG ACG AGC TCA AGA AGT TT -3′ (AS) 5′- AGC TCA GCG CTG TAC TCC TC -3′ |
154 bp | XM_003124468.4 | |
| IL-8 (interleukin-8, Sus scrofa) | (S) 5′- GGC AGT TTT CCT GCT TTC T -3′ (AS) 5′- CAG TGG GGT CCA CTC TCA AT -3′ |
154 bp | X61151 | [34] |
| TLR4 (toll-like receptor 4, Sus scrofa) | (S) 5′- AGA ACT GCA GGT GCT GGA TT -3′ (AS) 5′- AGG TTT GTC TCA ACG GCA AC -3′ |
180 bp | AB188301 | |
| TGF-β (transforming growth factor beta, Sus scrofa) | (S) 5′- TGA CCC GCA GAG AGG CTA TA -3′ (AS) 5′- CAT GAG GAG CAG GAA GGG C -3′ |
164 bp | NM_214015.2 | |
| TBP (TATA box binding protein, Sus scrofa) | (S) 5′- GAT GGA CGT TCG GTT TAG G -3′ (AS) 5′- AGC AGC ACA GTA CGA GCA A -3′ |
124 bp | DQ178129 | [35] |
| YWHAZ (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide, Sus scrofa) | (S) 5′- ATG CAA CCA ACA CAT CCT ATC -3′ (AS) 5′- GCA TTA TTA GCG TGC TGT CTT -3′ |
178 bp | DQ178130 | [35] |
| B2M (beta-2-microglobulin, Sus scrofa) | (S) 5′- AAA CGG AAA GCC AAA TTA CC -3′ (AS) 5′- ATC CAC AGC GTT AGG AGT GA -3′ |
178 bp | DQ178123 | [35] |
| GAPDH (glyceraldehyde-3-phosphate dehydrogenase, Sus scrofa) | (S) 5′- ACT CAC TCT TCT ACC TTT GAT GCT -3′ (AS) 5′- TGT TGC TGT AGC CAA ATT CA -3′ |
100 bp | DQ178124 | [35] |