Table 1.

Deletion of N- or C-terminal amino acids from 16-aa rat-derived SP-A peptide: effect on the inhibition of the aiPLA₂ activity of recombinant hPrdx6.

Peptide Length	Sequence Delete from N-term	Activity, nmol/min/mg Prot.	Sequence Delete from C-term	Activity, nmol/min/mg Prot.
control	No peptide	94.8	Scrambled peptide: LELDEEITEYQKQLHI	93.5
16 aa	DEELQTELYEIKHQIL	32.0	DEELQTELYEIKHQIL	32.0
14 aa	ELQTELYEIKHQIL	33.2	DEELQTELYEIKHQ	102
12 aa	QTELYEIKHQIL	31.5	No entry
10 aa	ELYEIKHQIL	28.6	No entry
9 aa	LYEIKHQIL (PIP-1)	32.3	ELYEIKHQI	89.6
8 aa	YEIKHQIL	94.4	DEELQTEL	93.6

Sequential deletions from the N-terminus or C-terminus of the rat/mouse 16-aa inhibitory peptide were used to determine the minimal effective sequence to inhibit the aiPLA₂ activity of peroxiredoxin 6 (Prdx6). The results represent one assay done in duplicate.