Fig. 1.

Indirect immunofluorescence analysis of salivary glands from adult female Anopheles gambiae of different ages. Anopheles gambiae Saglin-specific monoclonal antibody mAb2A3 was used to localize Saglin in salivary glands from females of various ages and physiological states (unfed, fed, pre- and 6 days post-oogenesis). The anti-Saglin antibody mAb2A3 was localized with an Alexafluor 555-labeled goat-anti-mouse secondary antibody and visualized using a fluorescence compound microscope. Salivary glands are shown in pairs of images, one taken using phase contrast optics and the other under fluorescence illumination. Without exception, Saglin was detected in the medial and proximal lateral lobes. No fluorescence was detected in the distal lateral lobes of the salivary glands