Fig. 3.

Superose 6 10/300 fractionation of HT:F-insv. His-Thioredoxin F-Insv (HT:F-Insv) was fractionated on a Superose 6 10/300 column, and the column fractions were then assayed for DNA-binding activity by EMSA using the 32-bp P3 probe. a DNA-binding activity elutes in a broad peak with an estimated molecular weight ranging from ~ 320 kDa to 120 kDa. Arrows indicate the different shifts that are generated by the HT:F-Insv protein. b Fractions from the gel filtration column, as indicated, were analyzed by SDS-PAGE, and the gel was stained with Coomassie to visualize HT:F-Insv