Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 3;12:2. doi: 10.1186/s13072-018-0249-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 7 — In vivo architectural functions of wild-type and mutant Insv proteins. A Adult abdominal cuticle preparations of a wild-type male. The fifth and sixth tergites are pigmented, and the A6 sternite is recognizable by the absence of bristles and a banana shape. Trichomes are visible in the dark field (black and white lower panels) and cover the entire surface of the A5 tergite, while for the A6 tergite the trichomes are found in thin stripes along the anterior and ventral edges of the A6 tergite. B Homozygous males with a sensitized genetic background of Fab-7: Fab-7^GAGA1-5 (for description see the text). C When the insv null allele, insv^23B, is combined with Fab-7^GAGA1-5, this disrupts the boundary function of the Fab-7^GAGA1-5 replacement and A6 is misspecified. Males have a mixed GOF and LOF phenotype. A6 is partially transformed into A7 (GOF transformation), but at the same time, the residual A6 cuticle has morphological features characteristic of A5 (trichomes visible in the dark field, LOF transformation). D Rescue of the mutant insv^23B; Fab-7^GAGA1-5 phenotype with a genomic rescue construct insv^+3.67. The genomic Insv fragment restores the boundary function of Fab-7^GAGA1-5. The A6 tergite is only marginally smaller than wild type, and the trichomes are usually limited to small patches. E–G Show the abdominal cuticles of males carrying the ubi rescue constructs in an insv^23B; Fab-7^GAGA1-5 background. E Wild-type ubi-insv^+3.67 rescues all Fab-7^GAGA1-5 boundary defects. Males have tergites and sternites that are indistinguishable from the wild type. F Rescue obtained with ubi-insv^d135-150 construct. Although insv^23B; ubi-insv^d135-150 Fab-7^GAGA1-5 homozygotes look almost wild type, A6 sternites typically have two or more bristles, indicative of a weak A6 to A5 transformation. G Rescue by the coiled-coil domain deletion transgene 5xpita-ubi-insv ^d50-79 is incomplete. The A6 tergites in insv^23B; 5xpita-ubi-insv ^d50-79 Fab-7^GAGA1-5 males are reduced in size and are irregularly shaped, as expected for a strong GOF transformation. The tergites also have small patches of trichome hairs in regions where they are absent in wild type, indicating that it is also a LOF transformation. Sternites are also much smaller than in Fab-7^GAGA1-5 and unlike Fab-7^GAGA1-5 are misshapen or absent altogether. The numbers 4, 5, and 6 indicate A4, A5, and A6 abdominal segments, respectively