Skip to main content
. 2019 Jan 1;33(1-2):55–60. doi: 10.1101/gad.319939.118

Figure 1.

Figure 1.

Ring1a/b double-knockout epithelium displays fragile skin epidermis. (A) Hematoxylin and eosin (H&E) staining. Bar, 25 µm. (B) IF staining for the basal layer marker KRT14 (red) and the differentiated suprabasal layer marker KRT10 (green). Bar, 25 µm. (C) IF staining for the late differentiation marker filaggrin (FLG; green). The basement membrane is labeled by ITGβ4 (red). Bar, 25 µm. (D) X-gal skin permeability assay in newborn Ring1a/b double-knockout and control mice. (E) TUNEL assay for apoptosis (green). Skin epithelium is labeled by E-cadherin (ECAD; red). Bar, 25 µm. (Epi) Epidermis; (Hf) hair follicle; (Der) dermis.