Skip to main content
. 2019 Jan 1;33(1-2):75–89. doi: 10.1101/gad.315978.118

Figure 6.

Figure 6.

ZPET inhibits resection by binding ssDNA and functions independently of 53BP1 and HELB. (A) HA-tagged ZPETWT, ZPETΔZF2-4, and ZPETΔZF3-5 were transiently expressed in HEK293T cells. Cells were treated with 1 µM CPT for 45 min or left untreated, and the levels of the indicated proteins were analyzed by Western blot. (B) HA-ZPET expression was induced by Dox in U2OS cells as indicated. Biotinylated ssDNA was used to capture proteins from cell extracts containing both chromatin-bound and soluble fractions. The levels of HA-ZPET and MRE11 on ssDNA and in input extracts were analyzed by Western blot. (C) Wild-type or 53BP1 knockout U2OS cells were transfected with control or ZPET siRNA as indicated. Cells were treated with 1 µM CPT for 45 min or left untreated. Cell lysates were analyzed by Western blot using the indicated antibodies. (D) Wild-type and ZPET knockout lines were transfected with either control or HELB siRNA as indicated and analyzed by Western blot using the indicated antibodies.