(a) Coronal sections of P0 brains stained with the layer II-IV marker
Cux1, the layer V marker Sox5, and the layer VI/subplate (SP) marker Tbr1.
Dashed white lines mark borders of Cux1+ and Sox5+ neuronal layers. (b)
Quantification of thickness of Cux1+, Sox5+, or Tbr1+ neuronal layers, one-way
ANOVA (n = 3 brain sections for all experimental groups; Cux1+,
P = 2.689E-07, F (2, 6) = 461.8; Sox5+,
P = 0.115, F (2, 6) = 3.169; Tbr1+,
P = 0.8865, F (2, 6) = 0.1229) followed by
Bonferroni’spost hoc test (Cux1+, WT vs. KO,
P = 4E-07, 95% C.I. = 84.39 to 105.9, WT vs. Het,
P = 0.9999, 95% C.I. = −10.97 to 10.52; Sox5+, WT
vs. KO, P = 0.1329, 95% C.I. = −14.18 to 101.2, WT vs.
Het, P = 0.9999, 95% C.I. = −55.32 to 60.06; Tbr1+, WT
vs. KO, P = 0.9999, 95% C.I. = −43.31 to 46.59, WT vs.
Het, P = 0.9999, 95% C.I. = −50.47 to 39.42). (c)
Coronal sections of P0 brains stained with the layer II-IV marker Satb2. (d)
Quantification of number of Satb2+ cells, one-way ANOVA (n = 3
brain sections for all experimental groups; P = 0.00015,
F (2, 6) = 53.83) followed by Bonferroni’s
post hoc test (WT vs. KO, P = 0.0003, 95%
C.I. = 198.2 to 408.5, WT vs. Het, P = 0.9186, 95% C.I. =
−133.1 to 77.14). (e) Coronal sections of E17.5 brains stained with Cux1;
dashed white lines mark border of Cux1+ neuronal layer. (f) Quantification of
Cux1+ layer thickness within dashed white lines and of the average number of
newly generated Cux1+ cells within 1 mm2, as measured from the VZ to
the lower dashed white lines, at E17.5. One-way ANOVA (n = 3
brain sections for all experimental groups; thickness, P =
0.0019, F (2, 6) = 21.36; number, P = 0.0004,
F (2, 6) = 36.75) followed by Bonferroni’s
post hoc test (thickness, WT vs. KO, P =
0.0025, 95% C.I. = 9.765 to 30.85, WT vs. Het, P = 0.9999, 95%
C.I. = −10.13 to 10.96; number, WT vs. KO, P = 0.0004,
95% C.I. = 181.5 to 401.9, WT vs. Het, P = 0.7499, 95% C.I. =
−74.64 to 145.8). Scale bars: 200 μM. Graphs represent the mean
± SD. Dots represent data from individual data points. ns =
non-significant. ** P < 0.01, *** P
< 0.001, **** P < 0.0001.